The DNA Fragment Selection Kit is designed for the purification and selection of DNA fragments in various genomic applications. It is suitable for the use in PCR/qPCR/ddPCR, NGS, gene chips, and other processes requiring the purification and selection of DNA fragments. This kit can besuit for DNA and RNA library preparation and well compatible with library preparation kits of many brands and laboratory developed. The kit used exactly in the same way as AMPure XP Beads, no need to try different conditions and highly comparable to AMPure XP Beads with regard to recovery rate and library sizes.

Based on SPRI technology (solid phase reversible immobilization), the kit utilizes high-performance magnetic beads and a unique buffer system to selectively purify and select DNA fragments within the range of 100 bp to 2,000 bp without DNA gel extraction or specialized equipment. By adjusting the ratio of reagents to samples, it effectively removes dNTPs, salt ions, primers, primer dimers, and other impurities from the reaction system. The resulting purified DNA fragments exhibit high purity levels with consistent fragment lengths that meet sequencing instrument requirements. This kit can be utilized for both manual operations or automated high-throughput workflows.

The Principle of Operation of DNA Fragment Selection Kit

In a solution containing a certain concentration of salt and PEG (polyethylene glycol), changes occur in the conformational structure of DNA molecules leading to an exposure of numerous negatively charged phosphate groups. Under the influence of salt ions present in the solution, these phosphate groups bind to carboxyl magnetic beads, higher concentrations result in smaller bound DNA fragments on these carboxyl magnetic beads.Once the specific adsorption occurs between the target DNA fragments and magnetic beads, they are separated from solution using an external magnetic field which eliminates PEG- and salt-containing solutions followed by further washing steps removing residual PEG- and salts from the magnetic beads. The addition of elution buffer allows recovery of selected and purified DNA fragments from the magnetic beads.

This product consists of salt solution containing carboxyl magnetic beads and PEG by controlling the reagent to sample volume ratio during experiments,the concentrations of PEG and salt in the solution can be managed,enabling the binding of DNA molecules of ten different lengths to the magnetic beads for selection and purification.This product follows an experimental process for selecting DNA fragment lengths and the procedure is illustrated below:

Strong Specificity & High Binding Capacity of DNA

This product enables rapid separation/purification of nucleic acid segments across different sizes without DNA gel extraction.It exhibits fast binding speed with high binding capacity. Additionally, the small particle size minimizes non-specific adsorption, resulting in efficient removal of dNTPs, primers, primer dimers, salts, and other contaminants.

Wide range of selection length (fragments 100bp-2000bp)

The product can adjust the range of selected DNA length by adjusting the amount of reagent which can meet the needs of different experiments. Results are consistent across different batches. The range of selected fragments is consistent with the experiment design and the experiment results are predictable.

High selecting efficiency

The product uses magnetic separation without centrifugation, which helps to retain the integrity of nucleic acid and the purification rate of DNA fragments is high.

1.Construction of DNA libraries

In genome sequencing, it is necessary to break the DNA into fragments of certain length, then amplify these fragments by PCR, and finally clone these fragments onto the vector to form DNA libraries. In this process, the utilization of fragment selecting magnetic beads enables the targeted enrichment of DNA fragments with specific lengths., thereby improving the quality of the library.

2.Genome re-sequencing

Genome resequencing is an important method to study genome variation, which can reveal the structure, function and evolution of the genome. In this process, the utilization of selecting magnetic beads enables the targeted enrichment of specific DNA fragments, thereby improving the accuracy and efficiency of sequencing.

3.Study on gene expression profile

Gene expression profile is a method to describe the expression of genes under different conditions, which can reveal the function and regulatory mechanism of genes. In this process, DNA fragment selection magnetic beads can be utilized for selective enrichment of specific types of RNA molecules, thereby improving the accuracy and depth of expression profiles.

4.Study on chromosome assembly and structure

Chromosome assembly refers to the reassembly of short read sequences obtained through sequencing into contiguous chromosome sequences, while the study of chromosome structure investigates the spatial organization and functional aspects of chromosomes. During this process, fragment selecting magnetic beads can be utilized for selective enrichment of specific DNA fragments, thereby enhancing the accuracy and efficiency of assembly and structural analysis.