According to the principle of nucleic acids isolation based on silica spin（Boom, 1990）, Superparamagnetic Silica Nanoparticles(also called magnetic silica beads, magnetic beads, beads）are manufactured by surface modification and improvement build on the magnetic nanoparticles by nanotechnology methods. The magnetic silica beads can bind nucleic acids with high specificity and efficiency. Due to the principle of nucleic acids adsorption and the superparamagnetism of magnetic silica beads, all types of nucleic acids can be isolated many materials, such as blood, tissues, foods, microorganism, In the presence of Chaotropic salt (e.g. guanidine hydrochloride, guanidine isothiocyanate). The principle of magnetic silica beads isolation nucleic acids is below.

Fig. Flowchart of DNA/RNA purification based on magnetic silica beads

AllMag® nucleic acids isolation kits are designed by using our patent superparamagnetic beads and distinct buffer system. The kits can extract high quality DNA/RNA from blood, tissues and forensic samples, without several extraction and centrifugation steps.  The purified nucleic acids by kits can be directly used for downstream application, such as enzyme restriction, sequencing, quantitative PCR and STR analysis. AllMag® nucleic acids isolation kits enable laboratories to automatically and conveniently isolate DNA/RNA from thousands of samples per day through automation equipments.

In combination with our patent superparamagnetic beads and distinct buffer system, AllMag® Blood DNA Kit can extract genomic DNA from fresh or frozen human whole blood, buffy coat and cells. The kit especially enables isolation of genomic DNA from variable amounts (10μl-10 ml) of whole blood. The purified DNA with high quality and without PCR inhibitors, and is ready to use for a broad range of downstream applications or can be stored at –20°C for subsequent use.

The kit is suitable for purification of genomic DNA with high throughput and automatically.

• Handiness and fast

Due to the high magnetite content, the binding complex of AllMag® magnetic silica beads and DNA is quickly separated under magnetic filed. The entire process is about 30min, and without several centrifugation steps. It is well suitable for forensic DNA isolation through automation.

• High DNA yield

AllMag® magnetic silica beads have higher binding ability with nucleic acids after surface modification and this account for high genomic DNA yield.

• High purification quality

Patented AllMag® magnetic silica beads having good resuspension and dispersity abilities, this makes beads can be washed easily and thoroughly, therefore the extracted DNA have high quality without salts and PCR inhibitors.

• High performance cost ratio

The steps of sample lysis and DNA binding can be done simultaneously and without Proteinase K, and the entire process is only about 30min.

• Safety and environmental friendly

No organic reagent such as alcohol phenol / chloroform, no special treatment for waste liquid

Genomic DNA isolation from different amount of blood

Genomic DNA isolated from 10μl, 50μl, 100μl, 150μl, 200μl and 300μl human whole blood using AllMag® Blood DNA Kit(A10901) with two replications. The DNA were eluted with 100μl elution buffer. The concentration of purified were calculated by NanoDrop-1000. 1μl of each eluted DNA from 100μl elution were analyzed on a 1 % TBE agarose gel stained with GelRed

Fig. DNA was purified from different amount of blood of human whole blood samples   using the AllMag® Blood DNA Kit(A10901).  Lane M: DNA Marker（Fermentas #SM0191）; Lane 1-6: blood volume are 10μl, 50μl, 100μl, 150μl, 200μl and 300μl respectively

Fig. The linear relation between the amount of purified DNA and the blood volume.

Best Reproducibility

AllMag®Blood DNA Kit(A10901) was used for extraction genomic DNA from 200μl human whole blood, and the experiment had eight replications. 1μl of each eluted DNA from 200μl elution were performed by PCR amplification. 2μl eluted DNA and 2μl PCR sample were analyzed on a 1 % TBE agarose gel stained with GelRed.

Table The concentration and purity of isolated genomic DNA(200μl elution)

Fig. DNA were isolated with the AllMagTM Blood DNA Kit(A10901), Lane M: DNA（Fermentas #SM0191）；Lane1-8: genomic DNA of eight parallel replication.

Fig. PCR amplification results of corresponding genomic DNA of eight parallel replication. Lane M: DNA Marker（TaKaRa D501A）; Lane 1-8: genomic DNA isolated by AllMagTM Blood DNA Kit; Lane 9: Positive control; Lane 10: Negative control.

Isolation genomic DNA from 1ml human whole blood

AllMag® Blood DNA Kit(A10901) was used for extraction genomic DNA from 200μl and 1ml human whole blood, and the experiment had four replications. Beads, all the reagents and elution volume are proportionally scaled up when extract DNA from 1ml human whole blood. 1μl of each eluted DNA from 100μl elution and 500μl elution were analyzed on a 1 % TBE agarose gel stained with GelRed.

Fig. Gel electrophoresis of isolated genomic DNA. Lane M: DNA Marker（Fermentas #SM0191）

Fig. DNA amount and purity of purified DNA form 200μl and 1ml blood.

Performance comparison

AllMag® Blood DNA Kit (A10901) was used for extraction genomic DNA from 200μl human whole blood, and the same kind kit from P Company as the control. Each test has four replications. The concentration and A260/280 of purified DNA were calculated by NanoDrop-1000.

Fig. DNA amount and purity of purified DNA form 200μl blood with AllMagTM Blood DNA Kit and P Company Kit.