According to the principle of nucleic acids isolation based on silica spin（Boom, 1990）, Superparamagnetic Silica Nanoparticles(also called magnetic silica beads, magnetic beads, beads）are manufactured by surface modification and improvement build on the magnetic nanoparticles by nanotechnology methods. The magnetic silica beads can bind nucleic acids with high specificity and efficiency. Due to the principle of nucleic acids adsorption and the superparamagnetism of magnetic silica beads, all types of nucleic acids can be isolated many materials, such as blood, tissues, foods, microorganism, In the presence of Chaotropic salt (e.g. guanidine hydrochloride, guanidine isothiocyanate). The principle of magnetic silica beads isolation nucleic acids is below.

Fig. Flowchart of DNA/RNA purification based on magnetic silica beads

AllMag® nucleic acids isolation kits are designed by using our patent superparamagnetic beads and distinct buffer system. The kits can extract high quality DNA/RNA from blood, tissues and forensic samples, without several extraction and centrifugation steps.  The purified nucleic acids by kits can be directly used for downstream application, such as enzyme restriction, sequencing, quantitative PCR and STR analysis. AllMag® nucleic acids isolation kits enable laboratories to automatically and conveniently isolate DNA/RNA from thousands of samples per day through automation equipments.

AllMag® Blood DNA Kit (Proteinase K)is designed for genomic DNA extraction from blood and tissue of human and mammalian, based on our patent superparamagnetic beads and distinct buffer system. The kit especially enables isolation of genomic DNA from variable amounts (10μl-10 ml) of whole blood. The purified DNA with high quality and without PCR inhibitors, and is ready to use for a broad range of downstream applications or can be stored at –20°C for subsequent use.

The kit is suitable for purification of genomic DNA with high throughput and automatically. 

• Handiness and fast

Due to the high magnetite content, the binding complex of AllMag® magnetic silica beads and DNA is quickly separated under magnetic filed. The entire process is about 15-20min after sample lysis, and without several centrifugation steps.

• High DNA yield

AllMag® magnetic silica beads have higher binding ability with nucleic acids after surface modification and this account for high genomic DNA yield.

• High purification quality

Patented AllMag® magnetic silica beads having good resuspension and dispersity abilities, this makes beads can be washed easily and thoroughly, therefore the extracted DNA have high quality without salts and PCR inhibitors.

• Safety and environmental friendly

No organic reagent such as alcohol phenol / chloroform, no special treatment for waste liquid.

• Best Reproducibility

AllMag® Blood DNA Kit(A10301) was used for extraction genomic DNA from 200μl human whole blood, and the experiment had eight replications. 1μl of each eluted DNA from 100μl elution were performed by PCR amplification. 1μl eluted DNA and 2μl PCR sample were analyzed on a 1 % TBE agarose gel stained with GelRed

Table The concentration and purity of isolated genomic DNA

Fig. DNA were isolated with the AllMag® Blood DNA Kit(A10301), Lane M: DNA（Fermentas #SM0191）；Lane1-8: genomic DNA of eight parallel replication

Fig. PCR amplification results of corresponding genomic DNA of eight parallel replication. Lane M: DNA Marker（TaKaRa D501A）; Lane 1-8: genomic DNA isolated by AllMag® Blood DNA Kit; Lane 9: Positive control; Lane 10: Negative control

• Performance comparison

AllMag® Blood DNA Kit (A10301) was used for extraction genomic DNA from 200μl human whole blood, and the same kind kit from Q Company as the control. Each test has four replications. The concentration and A260/280 of purified DNA were calculated by NanoDrop-1000. 1μl of each eluted DNA from 100μl elution were analyzed on a 1 % TBE agarose gel stained with GelRed

Fig. The amount and A260/280 ratio of purified DNA with AllMagTM Blood DNA Kit and Q Kit

Fig. Gel analysis result of purified DNA with AllMag®Blood DNA Kit and Q Kit（Lane M: DNA Marker（Fermentas #SM0191）; Lane 1-4: genomic DNA by AllMag® Kit; Lane5-8: genomic DNA by Q Kit）