According to the principle of nucleic acids isolation based on silica spin（Boom, 1990）, Superparamagnetic Silica Nanoparticles(also called magnetic silica beads, magnetic beads, beads）are manufactured by surface modification and improvement build on the magnetic nanoparticles by nanotechnology methods. The magnetic silica beads can bind nucleic acids with high specificity and efficiency. Due to the principle of nucleic acids adsorption and the superparamagnetism of magnetic silica beads, all types of nucleic acids can be isolated many materials, such as blood, tissues, foods, microorganism, In the presence of Chaotropic salt (e.g. guanidine hydrochloride, guanidine isothiocyanate). The principle of magnetic silica beads isolation nucleic acids is below.

Fig. Flowchart of DNA/RNA purification based on magnetic silica beads

AllMag® nucleic acids isolation kits are designed by using our patent superparamagnetic beads and distinct buffer system. The kits can extract high quality DNA/RNA from blood, tissues and forensic samples, without several extraction and centrifugation steps.  The purified nucleic acids by kits can be directly used for downstream application, such as enzyme restriction, sequencing, quantitative PCR and STR analysis. AllMag® nucleic acids isolation kits enable laboratories to automatically and conveniently isolate DNA/RNA from thousands of samples per day through automation equipments.

AllMag®Forensic DNA Kit can extract DNA rapidly and sufficiently from diverse and small forensic samples, such as blood spot, saliva, mouth swab and hair root, based on our patent superparamagnetic beads and distinct buffer system. The purified DNA with high quality and without PCR inhibitors, and has excellent performance in genotyping, sequencing, PCR and HLA typing. This kit is especially suitable for purification nucleic acid of trace amount, and can be operated automatically and manually.

• Handiness and fast

Due to the high magnetite content, the binding complex of AllMag® magnetic silica beads and DNA is quickly separated under magnetic filed. The entire process is about 15-20min after sample lysis, and without several centrifugation steps. It is well suitable for forensic DNA isolation through automation.

• Expert on trace amount of DNA isolation

AllMagTM magnetic silica beads have higher binding ability with nucleic acids after surface modification. Special buffer system design for forensic samples, ensuring the extraction of trace amount of target DNA from 1 hair root or 1×1mm2 blood spot.

• High purification quality

Patented AllMag® magnetic silica beads having good resuspension and dispersity abilities, this makes beads can be washed easily and thoroughly, therefore the extracted DNA have high quality without salts and PCR inhibitors.

• Safety and environmental friendly

No organic reagent such as alcohol phenol / chloroform, no special treatment for waste liquid.

• DNA isolation from small amount of human whole blood

AllMag® Forensic DNA Kit (Code No.A10401) and the same kind kit of P Company were used to isolate DNA from small volume of human whole blood according to the manual of each. DNA eluted by 50μl elution buffer. 15μl eluted DNA were analyzed on a 1.0 % TBE agarose gel stained with GelRed.

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Fig. The isolated DNA was analyzed by 1.0% agarose gel electrophoresis(Lane M: DNA Marker; Lane1-6: isolated DNA by AllMag® Forensic DNA Kit and P Company Kit) 

DNA isolation from blood spot

AllMag® Forensic DNA Kit (Code No.A10401) and the same kind kit purchased from D Company, P Company and I Company were used to isolate DNA from blood spot, and all process according to the manual of each. DNA eluted by 40μl elution buffer of each company. 1μl of each eluted DNA were performed by PCR amplification. 5μl PCR sample were analyzed on a 1.8 % TBE agarose gel stained with GelRed.

Fig. PCR amplification results of DNA isolated by different commercial Forensic DNA Kits. 

STR analysis of DNA purified from cigarette butts

DNA was isolated from a cigarette using the AllMagTM Forensic DNA Kit (Code No.A10401). Purified DNA was eluted and was used for STR test. The amplified products were analyzed on an ABI PRISMTM using ABI GeneScan and ABI Genotyper software.

Fig. STR test result of purified DNA by AllMag®Forensic DNA Kit from cigarette butt.