Description

Silica Beads（-OH）, short for AllMag®Superparamagnetic Silica Nanoparticles are designed for nucleic acid purification. These silica beads have a core-shell structure of magnetite-rich core and inorganic silica shell, with large numbers of silanol groups on the surface. They are available as aqueous suspensions and are ready to use after easy vortex. By mixing these silica beads with chaotropic salt（e.g. guanidine hydrochloride, guanidine isothiocyanate）, nucleic acids can be rapidly isolated from biological samples without centrifugation and organic extraction steps. These products enable laboratories to automatically and safely process tens of thousands of samples per day.

Applications

Extraction of genomic DNA from blood, tissue, bacteria, plant

Extraction of viral DNA/RNA 

Extraction of total RNA

Purification of PCR fragments 

Extraction of plasmid DNA

Flowchart of DNA/RNA purification based on silica beads

Performance

Binding capacity: 4-12µg genomic DNA per mg per 200µl whole blood( in AllMag®Blood DNA kit, Proteinase K reagent test results, the results will vary with the whole blood sample and the reagent system used

AllMag ® SM1-015 recommended usage: 1-2 mg/time

Storage and Stability

AllMag ® SM1 series magnetic beads are stable when stored at 2-10°C. Before use, please thoroughly vortex the suspension. Freezing,drying or centrifuging the AllMag®silica beads may result in irreversible aggregation and loss of binding activity. Before use, please thoroughly vortex the suspension.  

1.Excellent performance in viral DNA/RNA isolation and purification

2.Good resuspension and dispersity abilities, and increased binding efficiency

3.Reliable and stable application as well as excellent reproducibility 

4.Easy handling, and shortened assay development time

Viral DNA/RNA isolation

Fig. The performance comparison of HBV DNA isolation between SM1-015D beads and M beads (Competitor) from serum. The tests were evaluated in the Viral DNA/RNA Isolation Kit of K Company, and the corresponding DNA used for real-time PCR detection. The concentrations of HBV in serum were 50 IU/mL and 100 IU/mL.

Fig. The performance comparison of HCV RNA isolation between SM1-015D beads and M beads (Competitor) from serum. The tests were evaluated in the Viral DNA/RNA Isolation Kit of K Company, and the corresponding RNA used for real-time RT-PCR detection. The concentrations of HCV in serum were 1000 IU/mL and 5000 IU/mL.

High reproducibility in viral DNA/RNA isolation

Fig. The different batches of SM1-015D beads used for HBV DNA isolation comparing with M beads (Competitor). The tests were evaluated in the Viral DNA/RNA Isolation Kit of K Company, and the corresponding DNA used for real-time PCR detection. The concentration of HBV in serum was 100 IU/mL.

Fig. The different batches of SM1-015D beads used for HCV RNA isolation comparing with M beads (Competitor). The tests were evaluated in the Viral DNA/RNA Isolation Kit of K Company, and the corresponding RNA used for real-time RT-PCR detection. The concentration of HCV in serum was 1000 IU/mL.